Effect of inoculation density of somatic embryos for obtaining plantain ‘FHIA-21’ (AAAB) cultivars
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Abstract
embryogenesis in plantain and banana may become a
commercial propagation technology, due to the high
multiplication coefficient of somatic embryos. However,
it is important to get quality embryos with a synchronous
morphological development to achieve higher rates of
germination and plant conversion. Therefore, the aim of
this study was to determine the effect of inoculation density
of somatic embryos for obtaining plantain ‘FHIA-21’
(AAAB) cultivars. Then, four inoculation treatments were
studied: 0,2; 0,4; 0,6 and 0,8 grams of fresh mass (gMF)
of embryos on liquid culture medium of maturation. The
morphological and histological characteristics of somatic
embryos were evaluated after 30 days and transferred to a
semisolid germination culture medium. Results showed a
better synchronization in the morphological development
of somatic embryos when cultured with 0,6 Gmf, which
was evident by their length uniformity, the apical and root
meristem formation, as well as the accumulation of reserve
substances. Such embryos reached a higher percentage of
germination and whole plant formation, with statistical
differences compared to other treatments. These plants were
characterized by a longer pseudostem, more than two open
leaves and greater root number. This study is essential for
the mass propagation of plantain cv. ‘FHIA-21’ by somatic
embryogenesis.
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