Arbuscular mycorrhiza fungal inoculants of Glomus mosseae and Glomus sp1 in liquid suspension
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Abstract
evaluate the fungal vitality of Glomus mosseae (INCAM 2)
and Glomus sp1 (INCAM 4) in liquid suspension as well as its
biological characterization in transformed (Ri TDNA) carrot
rootlets (TCR). In the first one, a fungal viability of spores was
studied in a liquid suspension for preservation and distilled
water as a control for eight months was performed. Weekly
and monthly evaluations of viability averages were done. In
the second experiment, colonization capability of spores stored
for eight months in TCR was studied. Spore population,
germination average, and colonized root length were measured.
The corresponding ANOVA design and Tukey test for
comparing media was used. A higher viability and stability
after eight months were observed with important spore viability
losses in water media. Both strains showed its germinating
power without significant differences, up to 30 days of
incubation. Higher germination percentages in spores were
recorded, which could be related to the strong osmotic stress
occurred during the exposition time of spore wall in a liquid
suspension during eight months. During colonization tests, it
could be appreciated that spore population not only
germinated, but it had a profuse colonization in both strains 14
days later, that means liquid suspension was effective to keep
good functioning levels of the fungi studied, from the biological
viewpoint.
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