Mass microprogation of Stevia rebaudiana Bertoni in temporary immersion systems
Main Article Content
Abstract
a natural sweetener called “Stevia”. Its properties came from the diterpenoid glycosides presented in the leaves: a stevioside and rebaudisioside. The percentage of seed germination
of S. rebaudiana is very low and the plants produced are heterogeneous, so it is not suitable for mass propagation in the field.The tissue culture in temporary immersion systems, is an effective tool for micropropagation, since it increases the multiplication coefficient and produces the improvement in the quality of in vitro regenerated plants. This research determined the efficieness of propagation process using temporary immersion devices for scaling up the mass production of Stevia rebaudiana, with the use of liquid media in automated temporary immersion system (RITA®) and twin flasks system (BIT), compared with semisolid culture. Most of the treatments involving temporary immersion systems (BIT y RITA®), produced green plants and vigorous plants, with low levels of hyperhydricity. The highest average of regenerated leaves and shoots was obtained in the RITA® system, with 10 minutes of immersion every 8 hours. It was determined also that 10 minutes on inmersion each 12 hour with the BIT produced vigorous plants, with an increased length, fresh and dry weight mass. The use of
in vitro culture in temporary immersion bioreactors (RITA® and BIT), offers many advantages in the process of scaling the production of this kind of commercial plant, related to connentional propagation system and encouraging the process of acclimatization.
Article Details
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.