Isolation and characterization of Gluconacetobacter diazotrophicus strains

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Yoania Ríos Rocafull

Abstract

Gluconacetobacter diazotrophicus is an endophyte microorganism with direct and indirect mechanisms for vegetable growth promotion among its characteristics. Despite its great perspective to constitute the active principle of a bioproduct for agricultural purposes, so far there is not any product derived from this bacterial species in Cuba. The strain isolation and its characterization are important steps to obtain a biopreparation, since it enables the initial selection of strains with adequate characteristics for vegetable growth stimulation. In this research, 85 endophyte isolates were purified from different organs of 24 plant species. Four of them were identified as Gluconacetobacter diazotrophicus, when comparing its characteristics with those from pattern strains of the bacterial species. Microorganisms were selected from mango (Mangifera indica L.) and guava (Psidium guajava L.) fruits as well as from yucca (Manihot esculenta Crantz.) and beet (Beta vulgaris L.) stems. The presence of this microorganism was relevant in the first two crops for Cuba whereas the isolation in guava at the international level. The four strains had differences regarding their capacity to solubilize phosphorus, to produce indol acetic acid and its antagonistic activity against Fusarium moniliforme and Fusarium incarnatum. As strains were grouped according to their characteristics, mango and beet microorganisms were different, which are considered promising to study the effect of its interaction with other crops under in vivo conditions.

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How to Cite
Ríos Rocafull, Y. (2016). Isolation and characterization of Gluconacetobacter diazotrophicus strains. Cultivos Tropicales, 37(1), 34–39. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1149
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Original Article