Isolation and characterization of rhizobia from Crotalaria sp. in southern Ecuador

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Published: Apr 22, 2016
Keywords:
inoculation, legumes, nodulation, selection

Main Article Content

Francisco Guamán Díaz

Abstract

The present investigation was aimed to isolate and characterize native diazotrophic bacteria strains and assess their effect on nodulation and growth of Crotalaria sp. For the isolation, samples were taken from Crotalaria plants with root nodules, under different agro-climatic conditions of Loja province, Ecuador. A morpho-cultural, biochemical and physiological characterization of isolates was performed, also evaluating the effect of each isolate on the nodulation, growth and biomass of Crotalaria under greenhouse conditions. From these isolates, 10 strains with Rhizobial characteristics were obtained. All of them showed the ability to nodulate host plant. Results were remarkable in three isolates, due to a higher nodulation ability and efficiency on plant inoculation. These results are a starting point to obtain inoculants and its possible effect on Crotalaria plants used as N2 fixers under field conditions.

Article Details

How to Cite
Guamán Díaz, F. (2016). Isolation and characterization of rhizobia from Crotalaria sp. in southern Ecuador. Cultivos Tropicales, 37(1), 40–47. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1151
Issue
Vol. 37 No. 1 (2016)
Section
Original Article

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