Short communication. Modification of the staining method of polyphenoloxidases and carbonic anhydrases in plants
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Abstract
genetic diversity studies of different species. Therefore,
this study was aimed to describe an efficient histochemical
staining modification of carbonic anhydrase and polyphenol
oxidase isozymes in plants, which consists of replacing the
staining buffers used to develop these isozymes by distilled
water. The effectiveness of these proposed adjustments was
confirmed when using the “t” test of Student for related
data and conducting these tests in different crops. The
electrophoretic profiles observed in stainings showed the
same pattern of bands without significant differences among
treatments, demonstrating that it is possible to replace
traditionally used staining buffers in these techniques by
distilled water. The high repeatability of such stainings will
enable to employ the proposed modifications to develop
these isozymes.
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