Water effect deficit on morpho-physiological and biochemical changes in ‘MD-2’ micro-propagated pineapple plants at the end of acclimatization stage

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René C. Rodríguez-Escriba

Abstract

Current technology of pineapple micro-propagation has problems with the plants transition to field, these problems are associated to drastic changes of environmental conditions linked to poor hardening plant for such transit. A possibility of preparation is the induction of defense mechanisms to drought stress and get modulating CAM, so that the object of this paper was study plants grown for 30 days in two water status (well-watered plants and non-watered plants) after 30 days both groups were full-watered during 15 days. Measurements were made on D-leaf, in which were determinates the gas exchange, water-efficiency use (WEU), night CO2 uptake percentage, chlorophyll content, organic acids levels, succulence index (IS) and superoxide dismutase activity (SOD) (EC 1.15.1.1). After 15 days of drought, plants had the best response to field transfer, with increase in CAM expression, supported by the decline in total chlorophyll content and increases in the night CO2 uptake percentage, WEU, SI, and SOD activity. After 30 days of drought plants had a CAM strong response, with 100 % of CO2 uptake during night, but its rapid recovery with the establishment of irrigation to saturation of the substrate, showing high drought tolerance and great metabolic plasticity.

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How to Cite
Rodríguez-Escriba, R. C. (2016). Water effect deficit on morpho-physiological and biochemical changes in ‘MD-2’ micro-propagated pineapple plants at the end of acclimatization stage. Cultivos Tropicales, 37(5 ESP), 64–73. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1190
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Original Article