Efect of Pectimorf® on rooting and in vitro acclimatization of papaya (Carica papaya L.) shoots cultivar Maradol Roja
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Abstract
successfully reported by different researchers, however
the problems in the process of rooting and acclimatization
have not been completely resolved yet. The low percentage
of rooting and low survival of plants under conditions of
acclimatization remain a problem for the development
of an efficient micropropagation protocol for this crop.
Employment growth regulator cuban Pectimorf® could be
used to minimize this problem. In two experiments that were
conducted as treatments are made up of two concentrations
of sucrose (0 and 10 g L-1), two of auxin AIB (0 and 2 mg L-1)
and five Pectimorf® concentrations (3, 5, 7, 9 and 12 mg L-1).
Were used as controls: the culture medium rooting composed
of MS salts 50 %, 2 mg L-1 IBA, 0,4 mg L-1 thiamine, sucrose
40 g L-1 agar 7 g L-1 and the same culture medium without
sucrose and Pectimorf®, but with zeolite as carrier. The
results showed that the Pectimorf® had a positive effect
on rooting and in vitro acclimatization of papaya shoot.
The synergistic action of AIB with 9,0 mg L-1 Pectimorf®
allowed to obtain in vitro plants with greater leaf area, fresh
weight, number of roots, photosynthetic rate and stomatal
conductance, which together with a high percentage of
rooting and less percentage of open stomata allowed to reach
a 76,2 % survival ex vitro conditions.
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