Short communication Contaminant fungi in in vitro establishment of potato apexes

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Published: Sep 30, 2016
Keywords:
culture, Fusarium, Penicillium

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Sandra Pérez Álvarez
Instituto Politécnico Nacional, CIIDIR (Centro Interdisciplinario de Investigación para el Desarrollo Integral Regional), Unidad Sinaloa, Departamento de Biotecnología Agrícola, Blvd. Juan de Dios Bátiz Paredes No. 250, San Joachín, Guasave, Sinaloa, México, C.P. 81101

Abstract

Potato (Solanum tuberosum L.) is one of the most important food sources worldwide and it is affect for some pathogen either in the field or in the storage conditions. Some of the pathogens that attack this crop may remain in the tuber and they can be taken to the field at the time of planting so it is very important to obtain high quality potato seed for which in vitro culture is a useful technology. The aim of this work was the identification of fungal contaminants at the in vitro establishment phase of apexes of two potato varieties (Fianna and Atlantic). Minitubers were storage in the dark at 18 oC for 20 days to obtain apexes that were collected, disinfected and placed in Murashige and Skoog medium supplemented with sacarose and agar. After seven days of apexes establishment, 100 % of the samples showed contamination and damage inside, which were identified in an optical microscopy as Fusarium and Penicillium genera, both tuber pathogen. This research demonstrate once again the importance to have healthy and high quality material for in vitro culture and the absence of sanity in the material sold to the farmers to obtain pre-basic seed or G1.

Article Details

How to Cite
Pérez Álvarez, S. (2016). Short communication Contaminant fungi in in vitro establishment of potato apexes. Cultivos Tropicales, 37(4), 84–88. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1278
Issue
Vol. 37 No. 4 (2016)
Section
Original Article

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