Application of Mycelium Donor Plant (MDP) system on in vitro mycorrhization of potato

Main Article Content

Kalyanne Fernández Suárez
Stéphane Declerck
Loiret Fernández García
Eduardo Ortega Delgado

Abstract

In vitro mycorrhization of plants is a highly
complex process that depends on ensuring the needs of
plants and fungi, organisms with different nutritional
requirements. Today doesn´t exist an in vitro culture system
which guarantee the mycorrhization of Solanum tuberosum L.
(potato), although some authors have made several attempts
to achieve it. The aim of this study was to evaluate the
applicability of Mycelium Donor Plant (MDP) system
for in vitro mycorrhization of potato plants in order to get
mycorrhized plants in a short period, with proven practical
use. Experimentally, a similar system to MDP, in which
S. tuberosum plants (seven days old) were associated with
an extensive network of mycelium coming from Medicago
truncatula plants, previously mycorrhized with the AMF
Rhizoglomus intraradices was design. High levels of potato
plant colonization (55 %), just 12 days after contact the
mycelium networks, were obtained. Potato plants were in
excellent conditions to be transplanted and they were able
to reproduce the fungal colony when they were replanted
in fresh media, producing a large number of extraradicals
structures (mycelium and spores), demonstrating the
applicability of MDP system for in vitro mycorrhization of
S. tuberosum.

Article Details

How to Cite
Fernández Suárez, K., Declerck, S., Fernández García, L., & Ortega Delgado, E. (2017). Application of Mycelium Donor Plant (MDP) system on in vitro mycorrhization of potato. Cultivos Tropicales, 38(1), 31–38. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1337
Section
Original Article