Ecomic® y Licomic® (Glomus hoi like), biofertilizantes que no provocan daño genotóxico ni citotóxico en las células germinales del ratón CENP:NMRI
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Abstract
than the one of chemical synthesis, since it contributes to soil
and environmental preservation. This kind of work has been
developed at the National Institute of Agricultural Sciences
(INCA) by means of obtaining EcoMic® and LicoMic®
biofertilizers, Glomus hoi like stump, which implies several
predictive toxicity assays should be performed, with the
purpose of cancelling or minimizing the application of
compounds, whose risk/benefit rate would be undesirable for
society. Among the in vivo tests described for genotoxic
evaluation, there is an assay for the sperm cell head
abnormalities in mice. In order to evaluate the genotoxic
potential of both biofertilizers, Cenp:NMRI mice were treated
with three dose levels (20, 40 and 80 spores.mL-1) of each
product, besides a negative (Ringer solution in EcoMic® and
osmoprotector solution in LicoMic®) and a positive control
(Acrylamide 20 mg.kg-1 in both cases). Results from both
experiments show that the doses evaluated do not significantly
increase anomalous sperm percentage compared to the
negative control. Likewise, there was not a statistically
significant sperm quantity decrease compared to the negative
control. It was concluded the two biofertilizers evaluated do
not cause genotoxic or citotoxic effects on the mice´s
germinal cells treated.
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