Establishing a methodology for chromosomal counting in Spanish carnation (Dianthus caryophyllus L.)
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Abstract
species involves a high value, from the taxonomic point of
view, and it is very important for breeding as well as to detect
the genetic variability of in vitro culture. However, it is
impossible to perform a cytogenetic analysis without
previously establishing a procedure to distinguish and count
the chromosomes from a species; thus, the present study is
aimed at determining a method, which allows the adequate
visualisation and counting of chromosomes in Spanish
carnation (Dianthus caryophyllus L. var. Chabaud Red). 20
roots were randomly taken from pregerminated plants in a moist
chamber and submitted to several pre-treatments, different
hydrolysis times on HCl-1N and staining times on haematoxylin.
The chromosomes from 20 cells were counted per each
preparation, by using a Motic optic microscope connected to
a computer. It was possible to determine a method for
visualizing and counting chromosomes of Spanish carnation,
and to prove the chromosomal number of this species.
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