Methods used for parental selection in pre-breeding of plants

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Ayerin Carrodeguas-Gonzalez
Andrés Zúñiga-Orozco

Abstract

A key step in the pre-breeding stage of plant breeding is the selection of cultivars with desirable characteristics for use as parents to develop new crop varieties. With the development of technology, numerous laboratory techniques have emerged as key tools for breeding programs to save time and resources. For these reasons, the aim of this review was to describe techniques based on cytological and cytogenetic studies that are feasible for the selection of parents during the pre-breeding stage. Among the cytological techniques addressed in this review are: pollen viability and quality, stigmatic receptivity to know how long the stigma is receptive, and pollen tube growth to study incompatibility events. Another key step in pre-breeding is to determine the chromosomal number of the parents, which is possible by different techniques of classical cytogenetics (studies during mitosis and meiosis) and molecular cytogenetics (flow cytometry, Fluorescent in situ Hybridization and Genomic in situ Hybridization) that are discussed in this review.

Article Details

How to Cite
Carrodeguas-Gonzalez, A., & Zúñiga-Orozco, A. (2023). Methods used for parental selection in pre-breeding of plants. Cultivos Tropicales, 43(2), e15. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1666
Section
Review

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