Plant parasitic nematodes associated to selected accessions in Musa spp. Cuban germplasm collection
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Abstract
The field plant genetic resource banks are ex situ collections that contribute to the preservation of plant germplasm. In Cuba, a collection of Musa spp., a genus of plants that are affected by pests of phytoparasitic nematodes, is kept. The objective of the research was to determine the plant parasitic nematodes and the dominant generas associated with 22 selected accessions at the germplasm collection of Musa spp. in Cuba, kept at the Tropical Root and Tubers Research Institute (INIVIT, according its acronyms in Spanish). The accessions were ‘Burro CEMSA’, ‘Manzano Vietnamita’, ‘Pisang Ceilan’, ‘CEMSA ¾’, ‘INIVIT PV-2011’, ‘INIVIT PB-2012’', ‘Yangambí’, ‘Gran Enano’, ‘Gross Michel’, ‘Pisang Jari Buaya’, ‘Calcuta 4’, ‘FHIA-18’, ‘INIVIT PV-0630’, ‘Enano Guantanamero’, ‘Macho –’, ‘FHIA-21’, ‘SH 3436-L-9’, ‘FHIA-01’, ‘FHIA 01-V1’, ‘SH 3142’, ‘SH 3362’ and ‘FHIA-17’. Sampling was carried out and soil and roots were collected from accession and were transferred to Agricultural Nematology Laboratory at National Center for Plant and Animal Health (CENSA, according its acronyms in Spanish), where processed by Whitehead Tray and maceration-sifting methods. Extracted nematodes were killed and fixed with TAF. The identification of genera was made using taxonomic keys and populations by genus were quantified. The dominant genera were determined using the ecological indexes: frequency, density and prominence value. Fifteen genera of nematodes were identified in soil samples and nine in roots. The genera Meloidogyne and Helicotylenchus were predominant. The largest populations of Meloidogyne in roots were found in 'Yangambí' and 'FHIA-18'; while Helicotylenchus was present in higher populations in the accessions ‘INIVIT PV-0630’ and 'CEMSA ¾’.
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