Effect of an oligogalacturonide mixture on in vitro propagation of cassava (Manihot esculenta Crantz), var. CMC-40
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Abstract
Department of Genetics and Plant Breeding from the National
Institute of Agricultural Sciences (INCA), San José de las Lajas,
Havana, with the objective to obtain excellent cassava
(Manihot esculenta Crantz) vitroplants for acclimatization and
evaluate the effect of an oligogalacturonide mixture (DP 7-16),
known as Pectimorf®, on the culture medium employed for in
vitro micropropagation of cassava, in order to substitute or
minimize the concentrations of auxin naphtalene acetic acid
(NAA). Doses of 5, 10 and 15 mg.L-1 were used, besides the
culture medium proposed by INIVIT for in vitro growth and
rooting of cassava as control. After 21 days, leaf number, root
number, fresh and dry weights were evaluated as well as
vitroplant vigor. Results proved that it is feasible to substitute
NAA in the growing media by Pectimorf® at 10 and 15 mg.L-1.
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