Enzymatic and growth responses in a commercial variety of tobacco (Nicotiana tabacum, L.) treated with a chitosan polymer by foliar spray
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Abstract
different concentrations (0, 0.1, 0.5, 1, 2.5 g.L-1) of chitosan
polymer 30 days after planting (dap). 14 days after treatment,
variations caused by chitosan on plant growth were measured,
using the following variables: number of leaves, stem and roots
length and dry mass. Polymer treatments caused stimulation
and inhibition of the variables measured depending on the
chitosan concentration tested. Changes in the protein and
reducing carbohydrate contents were, also, obtain in tobacco
leaves sprayed with chitosan, mainly increments above
control at 37 dap, depending on the polymer concentration
tested. Nitrato reductase activity was just induced above
control at 37 dap with the highest concentration (2.5 g.L-1
)tested. Also, defense markers evaluated were just induced,
above control, at 33 dap, again depending on polymer
concentration. ? 1-3 glucanase activated at 1.0 g.L-1, while PAL
activated with the three highest concentration (0.5-2.5 g.L-1)
employed. According to results, there is no relation between
the increasing of defense responses and changes on proteic
and reducing carbohidrates content; however, due to the
complexity of the induced resistance response, it is necessary
to look deeple in to this study by including other markers and
different way of chitosan application.
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