Effect of the application of a spirostanic brassinoesteroid analog on banano (Musa spp.) plantlets during the acclimatization phase

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Humberto Izquierdo Oviedo

Abstract

The acclimatization of plantlets from in vitro
culture to ex vitro conditions is very important on bananas.
This study was carried out at the National Institute of
Agricultural Sciences and Plant-factory San Jose de las Lajas.
The objective was to evaluate the effect of a brassinosteroid
analog (ABr) applied during the acclimatization phase in some
morpho-anatomics, physiological and cytogenetic characters
in �??FHIA-18�?? clone vitroplants, obtained with the use of ABr
consecutively on each phase of in vitro culture of this
genotype. A mixture of organic matter and eutric compacted
Red Ferralitic soil at a rate of 3:1 v/v was used as a substrate.
At 45 days, survival percentage, total dry mass, soluble
proteins content, stomatal density, stomatal length and the
cuticle thickness was evaluated. As a result, vitroplants root
immersion for 15 minutes before being planted and foliar
spraying with ABr between 0.02-0.2 ?mol.L-1, 15 days after
planting, increased plant survival by 11 % approximately
compared to the control plants and they presented bigger foliar
area, dry mass and proteins content. It is informed for the first
time that ABr stimulates the thickness acclimatization of the
cuticle and maintains constant the chromosomes number.

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How to Cite
Izquierdo Oviedo, H. (2012). Effect of the application of a spirostanic brassinoesteroid analog on banano (Musa spp.) plantlets during the acclimatization phase. Cultivos Tropicales, 33(1), 71–76. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/28
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Original Article