Microsatellite characterization of guava (Psidium guajava L.) germplasm collection in Cuba
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Abstract
economically important fruit in Myrtaceae. Cuban breeding
program has been limited to the selection and introduction of
genotypes with promising agronomic characteristics, but
studies focussed on genetic diversity organization has not
been made, which is very important for the identification of
potential parents for breeding program. The utilization of
microsatellite markers for guava accession identification and
germplasm characterization was the main objective of this work.
A total of 34 different alleles ranging from three to seven were
detected and the average number of putative alleles per locus
was 4.57. Heterozygosity values ranged from 0.08 to 0.54 with
0.38 as the total average for this parameter. Except two
genotypes, all the accessions were differentiated as a result of
the molecular analysis and six diversity groups were detected,
showing an acceptable level of genetic variability in the
collection assayed. The high number of common alleles
detected suggests that most of the analyzed plant material
shares a common genetic ancestry. The microsatellites
evaluated will play an important role in the identification of
guava accessions representing an essential genepool for ex
situ maintenance. Furthermore, molecular genotyping detected
here will allow the efficient selection of parents for future guava
breeding programs.
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