Callogenesis and in vitro regeneration of rice (Oryza sativa L.) with the Cuban biostimulants Biostan and Liplant
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Abstract
Liplant on the in vitro callogenesis and regeneration of rice
(Oryza sativa L.) cvs. INCA LP-5, IACuba-28 and LC88-66
was studied, aimed to improve the amount and quality of the
material disposed for the genetic transformation in this species.
To study callogenesis, seeds were grown on a Murashige and
Skoog medium, supplemented or not with biostimulants, and
the percentage of callus formation, color, consistency and fresh
weight were evaluated at 28 days. In vitro plant regeneration
was studied six weeks after transferring calluses to KIBAN
medium, supplemented or not with biostimulants. The variables
evaluated were: callus regeneration ability, number of roots
and shoots. Biostan and Liplant were found to potentiate the
induction effect of 2,4-D on in vitro rice callogenesis. The
lowest concentrations of Biostan and Liplant favoured
INCA LP-5 regeneration, while biostimulants did not increase
regeneration in the remaining varieties with respect to the control.
Results suggest the use of these biostimulants
supplementing 2,4-D to obtain rice calluses for the genetic
transformation of this species.
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