Metabolic aspects of seedling growth and development of plantain (CEMSA ¾) micropropagated in temporary immersion bioreactors (BIT)
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Abstract
Bioreactors constitutes an effective tool for plant propagation;
it increases its multiplication coefficient and quality. Little is
known so far about the echophysiology of this new cultivation
technique, where plants are subjected to a liquid immersion
and the physiologic changes that take place during this stage
will be the responsible for plant quality. To evaluate the effects
of these conditions on the physiologic change of plantain
plants, a descriptive experiment was conducted during the
elongation phase. Enzymes related with carbon metabolism
measured plant heterotrophic behaviour during the studied
phase. High levels of acid invertase (AI) activities and
pyruvato kinase (PK) accompanied by low levels of sucrose
phosphate synthase (SPS) and phosphoenolpyruvate
carboxylase (PEPC) evidence these behaviours. The changes
induced by the atmosphere on net photosynthesis and
transpiration rates demonstrate the tendency of plants to use
a less autotrophy metabolism. The plants under in vitro
conditions do not totally depend on photosynthesis but rather
they present a photomixotrophic behaviour.
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