USE OF cDNA MICROARRAY TECHNOLOGY FOR IDENTIFICATION OF NOVEL GENES RESPONDING TO ABSCISIC ACID PHYTOHORMONE
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Abstract
powerful technology that will substantially increase the speed
of molecular biological research. This paper gives a survey of
DNA microarray technology and its use in gene expression.
Studies of 4370 unigenes from a library of rice endosperm and
leaf tissue were used to detect the expression levels of mRNA
from rice stem tissue treated by water and plant hormone ABA.
Results showed that the expression levels of five genes were
depressed by phytohormone ABA. Reverse Northern blotting
confirmed that one of the five genes H024g06 was really
depressed by ABA. Bioinformatic analysis showed that the
gene H024g06 is the same as the cytochrome C gene. Previous
researches revealed that the cytochrome C gene was related
to such stress responses as drought and coldness, while ABA
could induce plant stress response. Therefore, these results
suggest that the cytochrome C gene may have a certain
mediating function during the stress response induced by
ABA phytohormone.
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