The effect of MH-5 brassinosteroid analogue on in vitro rice plant (Oryza sativa L.) growth under water stress conditions
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Abstract
to know the MH-5 brassinosteroid analogue antistress
potentialities on in vitro rice plant growth subjected to short
water stress periods. Seeds from two varieties having different
tolerance to drought stress were cultivated on MS solid
medium at different MH-5 analogue levels (0, 10-4, 10-3, 10-2
and 10-1 mg. L-1). Fifteen days after inoculation, seed
germination was determined and plants were submitted to PEG
(MW 6000) solution simulating at -1.0 MPa water deficit for 0,
24 and 48 hours. Morphological characters as plant height,
number and length of root system, fresh and dry weights as
well as relative water content were evaluated. Results have
shown a positive stimulation on seed germination and the
response of some indicators by the effect of MH-5 analogue,
specially when plants were subjected to water deficit by 24
hours, where the greatest differences between varieties were
observed. The tolerant cultivar presented better results when
MH-5 at 10-4 concentration was added while the sensitive one
got them at 10-2 mg.L-1 concentration.
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