A new in vitro regeneration protocol in tomato (Lycopersicon esculentum Mill)
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Abstract
frequency plant regeneration of normal tomato plants. Dried
mature seeds of tomato (Lycopersicon esculentum Mill.) from
four cultivars were used to obtain adventitious shoot buds.
Sterilised seeds were sown on filter paper that had been wet
with water and precultured from zero to three days in the light
at 25oC. Precultured seeds were cut into two parts; the portion
consisting of the proximal part of the hypocotyls was cultured
on a medium with Murashige and Skoog salts, Gelrite 2g.L-1,
Mio-Inositol 100 mg.L-1, Tiamine 4 mg.L-1 , 3 % commercial
sucrose, without growth regulators. After two weeks, more
than 60 % of the elongated shoots were excised individually
from the explants and subcultured on the same medium for
rooting. At the same time, calli were subcultivated on a fresh
medium to obtain more adventitious shoots. Morphological
characteristics of regenerated plants and fruits were similar
and they set normally shaped fruits with mature seeds; on the
other hand, 40 regenerated plants showed no variations in
chromosome number (2n=24). The advantages of this
regeneration method are: it does not employ exogenous growth
regulators, it is feasibly handled, regenerated plants are
obtained in a short time and also whole plants were obtained
in other four cultivars applying this simple procedure. This
paper reports an efficient system for plant regeneration from
adventitious shoot buds.
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