Histological characterization of somatic embryogenesis in leaf explants of sweet potato (Ipomoea batatas L. Lam.)
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Abstract
Murashige and Skoog, supplemented with myoinositol
(100 mg.L-1), thiamin (1 mg.L-1), MS vitamin (10.0 ml.L-1), sucrose
(3 %), gelrite (0.2 %) and different growth regulators in the
culture medium, depending on the experimental objective, the
callogenesis and somatic embrygenesis were described in
explants from sweet potato leaves of INIVIT B 93-1 cv, through
a callus histological study; the site where cell proliferation
provided the induction of a endogenous callus was determined.
The callus could be seen after six days from the aseptic
inoculation. At the beginning, the callus was formed from
vascular bundles and later, due to the intense mitotic activity,
it began to occupy the whole mesophyll. This resulted in a
compact mass, destroying the explant epidermis and giving
birth to a heterogeneous yellow nodular mass outwards, and
five days later, appearing the embryogenic primordia that led
to somatic embryos after 20 days.
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