Isolation of proteolytic enzymes from pineapple harvest remains
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Abstract
destined to obtain proteases. Pineapple plants contain several
cysteine proteases, the major isolated component of the stem
is the so-called �??stem bromelain�?� (EC 3.4.22.32), which has
been traditionally used in food industry. However, in recent
years, there is a renewed interest for this enzyme after its high
antitumoral activity was reported. In the present study,
bromelain extraction was obtained and patented through an
experimental procedure. Yields of the optimized technology
were 20.8 g of product.kg-1 stems and 3.9 protein g.kg-1 stems,
with a specific activity of 1.36 U.mg-1. The isolated product
was very active (6 000 U.kg-1) and stable, with a majority
proteolytic fraction of 24 500 Da molar mass. The optimal pH is
7 and it is very stable in the pH range from 3 to 9 and
temperatures till 50°C. The lyophilized product maintained 82 %
of its activity at -20°C for one year.
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