Effect of brassinosteroid analogue (MH5) on Eucalyptus urograndis propagation in temporary immersion bioreactors
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Abstract
among the current techniques to reach the quick introduction
of new varieties in production. Eucalyptus urograndis (E.
grandis Hill ex Maiden x E. urophylla) plantlets were used to
increase explant quality in TIB and study the effect of plantlet
sizes on survival rates during acclimatization. The application
of low concentrations (0.01 and 0.1 mg.L-1) of the
brassinosteroid analogue MH5 in culture media during the
elongation stage increased plant height, leaf number, fresh
and dry weights in TIB. During acclimatization stage, the lowest
sized seedlings (1.1-2.0 cm high) reached the highest survival
rates (63 %) without statistical differences with 2.1-3.0 cm
classification. Growth variables (plant height, leaf number and
root number) got the highest values in the smallest plantlets
(1.1-2.0 cm), with significant differences just with leaf number.
Seemingly, it appeared that size factor had very little effect on
the survival of E urograndis seedlings during the
acclimatization process, rather than the quality of different
organs confirming it.
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