Effect of 2,4-diclorophenoxiacetic acid on embryogenic response of soybean cultivar INCASoy-27
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Abstract
of 2,4-dichlorophenoxiacetic acid (2,4-D) on the embryogenic
response of Cuban soybean cultivar INCASoy-27. Immature
zygotic cotyledons between 3,0-4,0 mm length were placed
with the abaxial surface on the culture medium for embryos
induction. The culture medium was formulated with
concentrations of 15, 25, 35, 40 and 45 mg.L-1 2,4-D. After four
weeks in culture medium, the number of embryogenic
cotyledons in the total of cotyledons and number of somatic
embryos by embryogenic cotyledon was quantified. The
percentage of immature zygotic cotyledons that produced
somatic embryos varied between 23-93 % with 2,4-D
concentration in the culture medium. The average number of
somatic embryos formed per immature cotyledon fluctuated
from 1,2 to 7,9 while in embryogenic cotyledons was 5,3 to
8,5 somatic embryos. The major response of this morphogenetic
route was obtained at a concentration range of 40 and 45 mg.L-1
2,4-D in the culture medium. A differentiation process of somatic
embryos with asynchrony in morphology began in the fourth
week of culture.
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