ISOENZYMATIC ANALYSIS FOR DETECTING In Vitro VARIABILITY AND/OR STABILITY OF ECONOMICALLY IMPORTANT CROPS
Main Article Content
Abstract
tissue culture techniques is the loss of genetic stability, because
micropropagated materials under these conditions usually produce
non-normal regenerating plants or somaclonal variants
of the original varieties. However, sometimes, much advantage
from these genetic disorders is taken in different plant breeding
programs. It is, for this reason, important to monitor genetic
stability of the material obtained, isoenzymatic techniques
being very useful for this purpose. The objective of this work
was to study the stability and/or genetic variability generated
under these conditions. For doing so, electrophoretic analyses
were performed to different enzymatic systems coming from
potato embryogenic calluses, as well as to leaf tissue of tomato,
soybean and coffee vitroseedlings, regenerated under in vitro
conditions. The analysis was carried out using polyacrilamide
gels at different concentrations, depending on the revealed
enzymatic system, using a Mighty Small II vertical
electrophoresis unit from �??Pharmacia Biotech�?�. With the use
of isoenzymes, monomorphism was detected in tomato, coffee
and soybean electrophoretic patterns, which allowed
corroborating the genetic stability in these crops. On the other
hand, potato calluses showed certain variations in the
electrophoretic patterns of peroxidases and esterases; by means
of such systems, it is feasible to detect any possible somaclonal
variant in this crop. The results also allowed corroborating the
importance of using such techniques as an auxiliary tool for
carrying out in vitro regeneration processes, as well as their
application for monitoring genetic stability in other
micropropagated vegetable species under these conditions.
Article Details
Those authors who have publications with this journal accept the following terms of the License Attribution-NonCommercial 4.0 International (CC BY-NC 4.0):
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material
The licensor cannot revoke these freedoms as long as you follow the license terms.
Under the following terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- NonCommercial — You may not use the material for commercial purposes.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
The journal is not responsible for the opinions and concepts expressed in the works, they are the sole responsibility of the authors. The Editor, with the assistance of the Editorial Committee, reserves the right to suggest or request advisable or necessary modifications. They are accepted to publish original scientific papers, research results of interest that have not been published or sent to another journal for the same purpose.
The mention of trademarks of equipment, instruments or specific materials is for identification purposes, and there is no promotional commitment in relation to them, neither by the authors nor by the publisher.