EVALUATION OF TWO BRASSINOSTEROID ANALOGUES ON CALLUS FORMATION AND SHOOT INDUCTION IN SWEET POTATO (Ipomoea batatas (L.) Lam.)
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Abstract
medium with growth regulators 2,4-D (0.01 mg.L-1), 6-BAP (0.2 mg.L-1)
and brassinosteroid analogues Biobras-6 (BB-6) or MH-5 at
different concentrations (0.001, 0.01, 0.05 and 0.1 mg.L-1) and
the brassinosteroid analogues used only or combined with
2,4-D in the medium for callus induction: Callus percentage
was evaluated after four weeks. For studying the influence of
selected analogues on shoot induction, four-week-old calli
from control medium (2,4-D + 6-BAP) were used and transferred
to media containing BB-6 or MH-5, either alone or combined
with IAA (0.05 mg.L-1) or with IAA (0.05 mg.L-1) and Kinetine
(0.2 mg.L-1). Calli were kept in incubation chamber under
sunlight conditions, at the temperature of 25 ± 20C, 80-90 %
relative humidity and 72-90 ?mol.m-2.S-1 light intensity. After
four weeks, different quantitative and qualitative aspects were
evaluated in calli and shoots obtained. The best results were
recorded when using growth regulators 2,4-D (0.1 mg.L-1) and 6-
BAP (0.2 mg.L-1) alone in the medium, as well as combined with
BB-6 or MH-5 brassinosteroid analogues at 0.01 mg.L-1
concentration. Shoot induction was favored, in general, when
brassinosteroid analogues were combined with IAA (0.05 mg.L-1)
and Kinetine (0.2 mg.L-1), compared to separate analogues in
the culture medium.
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