Bradyrhizobium elkanii ICA 8001 GUS A: A NEW STRAIN TO EVALUATE NODULATION GENE EXPRESSION
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Abstract
encoding �?-glucuronidase enzyme, is used in this work to
genetically transform Bradyrhizobium elkanii ICA 8001 strain
by a triparental mating conjugation. The conjugates obtained
were tested by a nod expression test and confirmed by
nodulation plant experiments. From 10 conjugates obtained,
only two colonies expressed GUS activity and only one of
them corresponded with Bradyrhizobium. A new strain was
obtained: B. elkanii ICA 8001 transformed with gus A in its
chromosome. The Nod A-gus expression analysis is compared
with the nodulation factor synthesis for three different culture
media. This result constitutes an important tool to study
nodulation genes in the most used strain in Cuban inoculants
for soybean.
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