In vitro establishment of taro (Xanthosoma sagittifolium Schott) apex
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Abstract
spp.) is frequently affected by microorganisms, which can
provoke delays of vitroplantlet production. The objective of
this paper was to establish saprophyte microorganism-free
taro in vitro cultural conditions, as well as the most effective
culture medium for establishing taro from virus-free apex.
Therefore, a superficial bud disinfection with sodium
hypochlorite as well as a treatment with Oxitetracyclin and
Eritromycin for a week was perfomed in the culture medium.
Six culture media were compared with the commonly used
check at taro initiation. The best medium was that with BAP
1 mg.L-1), IBA 0.3 mg.L-1, silver nitrate 5 mg.L-1 and sodium
tiosulphate 5 mg.L-1, which induced 65.2 % multiplication
increase, compared to the check.
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