Partial purification and characterization of xylanase from the commercial enzymatic preparation Novoban 240

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Published: Jul 5, 2013
Keywords:
hydrolases, ? xylanases, pathogenesis, enzymes, purification

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Dalila Paz-Lago
Instituto Nacional de Ciencias Agrícolas

Abstract

Novoban 240 is a commercial enzymatic preparation from Novo Industri A/S with amylase activity which is obtained by submerged fermentation of Bacillus amilolichuefaciens and is used in wine industry. Moreover, it possesses endoxylanase activity, a closely related enzyme with the hypersensitive response of plant tissues upon pathogenic attack. A methodology for the partial purification of this enzyme using ion exchange chromatography on DEAE cellulose and SP Sephadex C-25 is established. The discrete enhance of reducing power during the enzymatic hydrolysis is an indicator confirming the presence of an endoxylanase in Novoban 240, which might be used to obtain xylo-oligosaccarides with
inducing activity of plant defense. The molecular weight estimated for this enzyme by polyacrylamide gel electrophoresis is below 14 kDa.

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How to Cite
Paz-Lago, D. (2013). Partial purification and characterization of xylanase from the commercial enzymatic preparation Novoban 240. Cultivos Tropicales, 21(2), 27–31. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/751
Issue
Vol. 21 No. 2 (2000)
Section
Original Article

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