Acclimatization of sugarcane (Saccharum sp. hybrid) seedlings derived from temporary immersion systems

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Published: Jul 5, 2013
Keywords:
sugarcane, in vitro culture, physiological adaptation, illumination, survival, environmental control

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R. Rodríguez
UNICA

Abstract

Sugarcane plantlets (Saccharum sp. hybrid) var. C-91-301 micropropagated by temporary immersion systems (TIS) were used as crop material to study their behavior during acclimatization phase, employing a substrate source of filter cake + ash mixtures (1:1, v:v). In the first experiment, the influence of different photosynthetic photon flux densities (PPFD) was studied on survival, growth and development of plantlets. The best results were obtained when a high intensity light was used. In a second experiment, plantlets were placed under the controlled conditions of a relative humidity, light (PPFD) and temperature in greenhouses with automated irrigation system. The best results of every parameter  evaluated were achieved when outdoor conditions were managed. In the third experiment, at the beginning and every seven days until 42 days, fresh and dry weights, leaf and root numbers were evaluated. The behavior dynamics of each one confirms the increasing use of light and gradual decrease of relative humidity as an efficient management during acclimatization, firstly expressed by a higher survival rate to 95% and notable increases of plantlet growth and development. In the process, an increase in stomatal density and leaf area was observed differently from the trend evaluated in chlorophyll contents. The photosynthetic activity of plantlets under natural light conditions makes growth less dependent upon
agrotechniques in this phase.

Article Details

How to Cite
Rodríguez, R. (2013). Acclimatization of sugarcane (Saccharum sp. hybrid) seedlings derived from temporary immersion systems. Cultivos Tropicales, 21(3), 51–56. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/778
Issue
Vol. 21 No. 3 (2000)
Section
Original Article

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