Effect of MH5 brassinosteroid analog and kinetine on biomass formation in Coffea canephora var. Robusta calluses

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Published: Jul 5, 2013
Keywords:
brassinosteroid analog, kinetine, callus, biomass, Coffea canephora

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Diana García
Instituto Nacional de Ciencias Agrícolas

Abstract

With the objective of knowing the action of MH5 brassinosteroid analog and different concentrations of kinetine on the fresh and dry matter production in callus of Coffea canephora var. Robusta formed from foliar explants, a study was carried out in which different combinations of treatments and times of exposure were evaluated. Thus, the concentrations of 2, 1, 0.5 and 0.2 mg.L-1 kinetine and 0.1, 0.05 and 0.01 mg.L-1 MH5 were used. Data were analyzed by simple classification ANOVA and also factorially. On the other hand, the proportions of mass increase were calculated for the different treatments and their respective variances. The results showed that the former treatments with the smallest concentrations of kinetine allow to obtain the maximum values for both dry and fresh matters, when the calluses are placed in media with the presence of the smallest concentrations of MH5; in the same way, the behavior of proportions of increase was determined by the hormonal balance. Also, there was a decrease in the value of estimated variances in those treatments with the brassinosteroid analog.

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How to Cite
García, D. (2013). Effect of MH5 brassinosteroid analog and kinetine on biomass formation in Coffea canephora var. Robusta calluses. Cultivos Tropicales, 21(3), 39–45. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/779
Issue
Vol. 21 No. 3 (2000)
Section
Original Article

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