Effect of Thidiazuron upon embryo formation and plantlet regeneration in recalcitrant calluses of pineapple anthers

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Published: Jul 5, 2013
Keywords:
anther culture, embryos, seedlings, pineapple, Ananas comosus, Thidiazuron

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R. Benega
Universidad de Ciego de Avila (UNICA)

Abstract

In order to try to obtain haploid plants, results about the effect of Thidiazuron upon embryo formation and plantlet regeneration on recalcitrant calluses achieved from anther culture of pineapple (Ananas comosus (L.) Merrill) were presented. Calluses from different genotypes were cultured on Murashige and Skoog�??s medium containing several concentrations of plant growth regulators. There was a differential response among all tested genotypes and the kind of plant growth regulators employed upon androgenic embryo formation and plantlet regeneration. The best responses were obtained using 1.5 mg.L-1 Thidiazuron on Red Spanish �??Pinareña�??, Smooth Cayenne c.v. �??Oriente�?? and �??Piña blanca�?? respectively. Calluses from Smooth Cayenne c.v. �??Serrana�?? and Smooth Cayenne cv �??Mexico�?? did not respond to these culture
conditions.

Article Details

How to Cite
Benega, R. (2013). Effect of Thidiazuron upon embryo formation and plantlet regeneration in recalcitrant calluses of pineapple anthers. Cultivos Tropicales, 21(3), 47–50. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/782
Issue
Vol. 21 No. 3 (2000)
Section
Original Article

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