GENETIC DIVERSITY ANALYSIS IN RICE MUTANTS USING ISOZYME AND MORPHOLOGICAL MARKERS

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Published: Jul 8, 2013
Keywords:
isozymes, agromorphological traits, genetic diversity, rice

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Alba Alvarez
Centro de Estudios Aplicados al Desarrollo Nuclear (CEADEN)

Abstract

In this work, isozyme and agromorphological variability of radio-induced rice mutants with different cytoplasm bases was surveyed. Agromorphological data (plant type, lodging resistance, maturity and yield) were transformed into binary data. These markers, along with isozyme (Peroxidases, Esterases, Catalases, Alcohol Dehydrogenases and Polyphenoloxidases) data, were considered for genetic diversity analyses in order to estimate the extent of diversity generated by ionizing radiation in rice. The genetic similarity between genotypes was obtained based on Dice�??s Coefficient. The UPGMA phenogram defined three main clusters that clearly corresponded to the different rice cytoplasm sources. A bootstrapping analysis was performed to estimate the strength of the groups in the phenogram. According to their bootstrap value, Basmati-370 mutant lines could be considered statistically different from their mother variety. A Multiple Correspondence Analysis (MCA) showed a genotype dispersion around the three principal axes of variation. In general, the UPGMA phenogram pattern was corroborated at MCA. Variables such as: maturity, presence of Est-a and Prx-m bands and the absence of Est-i, Prx-h and Prx-i accounted for the highest contribution to variation. The adequacy of morphological and isozyme descriptors for the new mutant line validation is also discussed.

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How to Cite
Alvarez, A. (2013). GENETIC DIVERSITY ANALYSIS IN RICE MUTANTS USING ISOZYME AND MORPHOLOGICAL MARKERS. Cultivos Tropicales, 21(4), 39–44. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/792
Issue
Vol. 21 No. 4 (2000)
Section
Original Article

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