Effect of growth bioregulators on the somatic embryogenesis of Cleopatra (Citrus reshni Hort. ex Tan.) mandarin
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Abstract
embryogenesis is an indispensable prerequisite, to implement
some biotechnological tools in plant genetic improvement and
even more in the case of citrus. To increase the amount of
these crops in plantations, the in vitro propagation techniques,
such as somatic embryogenesis, can be used, which require
artificial culture media and phytohormones. Due to the high
costs of plant hormones, a Cuban alternative is the use of
national growth bioregulators, such as Pectimorf
®
and
brassinosteroid analogues: Biobras-6 and MH5. These
bioregulators, like the 24-Epibrasinolide, are effective in
morphogenetic processes, usually at low concentrations, to
substitute or complement auxins and cytokinins. The technique
for growing in vitro embryos was employed, to obtain an
embryogenic cell line of Citrus reshni Hort. ex Tan and, as an
embryo source, the ovaries of this species were taken, which
came from trees of Citrus station of Ceiba del Agua, Caimito,
Havana province. To maintain the embryogenic line, the
embryogenic calluses from the established cell line were used.
This work was aimed at evaluating the effect of Pectimorf
®
and
the brassinosteroids MH5, Biobras-6 and 24-Epibrasinolide,
to obtain and keep the embryogenic cell line of Cleopatra
mandarin (Citrus reshni Hort. ex Tan.). Results proved an
increased fresh weight of embryogenic callus in a shorter
cropping time by means of these bioregulators.
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