In vitro and in vivo mycorhization of potato (Solanum tuberosum var. Alfa) seedlings
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Abstract
plants develop make them more susceptible to
drought when transplanted. Arbuscular mycorrhizal fungi
contribute to plant growth and survival by reducing the stress
associated to nutrition, water relations, soil structure, pH, salts,
toxic metals and pathogens, so that they are considered
extremely useful as inoculants during the different stages of
plant micropropagation. Taking these aspects into account,
this work was developed to evaluate the mycorrhizal
inoculation feasibility during in vitro and in vivo stages of
potato (Solanum tuberosum L var. Alfa) crop micropropagation
process. Therefore, mycorrhizal inoculation was studied on
potato plants under in vitro growing conditions, with different
culture media (MS; M and a new medium N), with a randomized
complete design (RCD) and potato plants inoculated, when
transplanted to adaptation stage, by three single Glomus
species and two concentrates of AMF species (forest and
desert) on two different growth substrates using a bifactorial
arranged RCD. Positive effects were obtained on in vitro plants
inoculated in the M medium, even though it is evident to look
for new nutritionally-balanced media that guarantee both plant
growth and an efficient mycorrhizal establishment. Besides, at
the adaptation stage, there was a highly positive strain
inoculation response, showing a differentiated behaviour
according to the substrates, which was appreciated in all
variables studied (nutritional, fungal and physiological), so
proving the feasibility of using such microorganisms at this
potato micropropagation stage.
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