Quantification of the biological fixation of nitrogen in Canavalia ensiformis by the methods of natural abundance of 15N and difference of total N

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Published: Apr 10, 2017
Keywords:
green manures, coinoculation, biological fixation of the nitrogen, mycorrhizae, Rhizobium

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Gloria M. Martín Alonso
Instituto Nacional de Ciencias Agrícolas (INCA)
Yonger Tamayo Aguilar
Facultad Agroforestal de Montaña, Universidad de Guantánamo
Ionel Hernández Forte
Instituto Nacional de Ciencias Agrícolas (INCA)
Mario Varela Nualles
Instituto Nacional de Ciencias Agrícolas (INCA)
Ednaldo da Silva Araujo
Embrapa Agrobiología

Abstract

In this work were traced as objectives to evaluate the growth and N accumulation in Canavalia ensiformis, grown in a Cambisol soil of El Salvador municipality, county of Guantánamo, coinoculated with four isolated of Rhizobium and two strains of HMA, as well as to quantify the process of nitrogen simbiotic fixation of this species, by the methods of the natural abundance of 15N and the difference of total N. The dry mass growth and the N absorption of the jackbean sowed in monoculture were superior in presence of the isolated of Rhizobium Can 5, coinoculated with Rhizoglomus intraradices, in these plants, the BNF oscillated from 59 to 72 kg ha-1 in the dry and rainy period, respectively. The methods of BNF quantification, difference of total N and natural abundance of 15N were similar to each other, although the difference of total N overestimates the quantification values regarding the natural abundance.

Article Details

How to Cite
Martín Alonso, G. M., Tamayo Aguilar, Y., Hernández Forte, I., Varela Nualles, M., & da Silva Araujo, E. (2017). Quantification of the biological fixation of nitrogen in Canavalia ensiformis by the methods of natural abundance of 15N and difference of total N. Cultivos Tropicales, 38(1), 122–130. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/1347
Issue
Vol. 38 No. 1 (2017)
Section
Original Article

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