Coinoculation of rhizobia and Arbuscular Mycorrhizal Fungi in two common bean cultivars
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Abstract
The work was carried out with the objective of determining the best combination of rhizobia and arbuscular mycorrhizal fungi strains in two bean cultivars: CC25-9 black and Lewa in a Nitisol soil. It was used in a randomized block design. Using a bifactorial arrangement, the factors under study were: four levels of rhizobia: three strains and a control without inoculation and four levels of mycorrhizal inoculation: two strains and a control without inoculation, for a total of twelve treatments. The variables evaluated were: height of the plant, diameter of the stem, dry mass of the leaves and the stem, yield and its components. The results in the yield of the crop, showed that the best combinations for the cultivar CC 25-9 are INCAM 4+CIAT 899 and INCAM 11 with CF 1 and CIAT 899 and although these results did not differ from the rest of the coinoculated treatments, they were superior to the simple inoculation of the microorganisms. In the Lewa cultivar, the best combination was INCAM 4+CIAT 899, although without differences with INCAM 4+CF 1; INCAM 11 combined with the three rhizobia strains and the simple inoculation of CIAT 899. The effectiveness of the simple inoculation of the AMF strains INCAM 4 and INCAM 11 was different, it was depend on the cultivars under study. The degree of effectiveness of the simple inoculation of the rhizobia also depended on the cultivar, with CIAT 899 behaving as the best strain for the Lewa cultivar, while in the CC 25-9 N cultivar the three strains behaved similarly. The most effective coinoculation turns out to be INCAM 11+CIAT 899, INCAM 11+CF 1 and INCAM 4+CIAT 899, this last combination being the most efficient for cultivar Lewa.
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References
Colás, A., Díaz, B, Rodríguez, A., Gatorno, S. y Rodríguez, O. Efecto de la biofertilización en la morfof isiología y rendimiento del frijol común (Phaseolus vulgaris L.). Ctro. Agríc. 45: 34-42.2018.
Chazan, M. World Prehistory and Archaeology: Pathways through. Time Pearson Education, Inc. 2008. ISBN 0-205-40621-1.
Rivera, R.; Calderón, A.; Nápoles, M.C.; Ruiz, L.; Simo J.; Plana R. La validación a escala productiva del biofertilizante EcoMic® y su aplicación conjunta con rizobios en el cultivo del frijol, en centro y occidente del país. 2012. https://scholar.google.com.cu/scholar?q=Rivera+2012+validacion+productiva+del+biofertilizante&hl=es&as_sdt=0&as_vis=1&oi=scholar#d=gs_qabs&t=1656518913656&u=%23p%3Dbf9PtcqgG20J
ONEI (Of icina Nacional de Estadística e Información). Anuario Estadístico de Cuba 2018. 2019. pp. 6-32. en: Agricultura. La Habana, Cuba. http://www.one.cu/aec2017/09%20Agriculhtura.
Martínez, R.; Dibut. B. Biofertilizantes bacterianos. Editorial Científico-Técnica, La Habana, Cuba. 2012. 279p. ISBN 978-959-05-0659-8.
Wilches, W; Ramírez, M; Pérez, U; Serralde, D; Peñaranda A. y Ramírez, L. Association of arbuscular mycorrhizal fungi (AMF) with sugarcane plants (Saccharum off icinarum) for panela production in Colombia. Terra Latinoamericana 37. 2019. 175-184. doi: https://doi.org/10.28940/terra.v37i2.481
Martín, G.M.; Y. Tamayo, I.; Hernández, M.; Varela y da Silva E. Cuantificación de la fijación biológica de nitrógeno en Canavalia ensiformis crecida en un suelo pardo mullido carbonatado mediante los métodos de abundancia natural de 15N y diferencia de N total. Cult. Tropic. 2017.38: 122–130.
Hernández, A.; Pérez, J.; Bosch, M.; Castro, D. Nueva versión de clasif icación genética de los suelos de Cuba. Ediciones INCA. Mayabeque, Cuba. 2015.91p..ISBN: 978-959-702377-7.
Paneque, V. M.; Calaña, J. M.; Calderón, M.; Borges, Y.; Hernández, T. C. y Caruncho, M. Manual de técnicas analíticas para análisis de suelo, foliar, abonos orgánicos y fertilizantes químicos. Ediciones INCA. 2010. 160 p, ISBN: 978-959-7023-51-7.
Zhang, S.; Lehmann, A.; Zheng, W.; You, Z.; Rillig, M.C. Arbuscular mycorrhizal fungi increase grain yields: a meta-analysis. New Phytologist. 2018.doi: 10.1111/nph.15570
Rillig, M.C.; Aguilar-Trigueros, C.A.; Camenzind, T.; Cavagnaro, R.T.; Degrune, F.; Hohmann, P. et al.,Why farmers should manage the arbuscular mycorrhizal simbiosis? New Phytologist. 2019. 222(3): pp 1171-1175. DOI: 10.1111/nph.15602.
Santos, M.S.; Nogueira, M. A.; Hungria M. Microbial inoculants: reviewing the past, discussing the present and previewing an outstanding future for the use of beneficial bacteria in agriculture. AMB.2019.Expr. https://doi.org/10.1186/s13568-019-0932-0
Rivera, R.; Fernández, F.; Ruiz, L.; González, P.J.; Rodríguez, Y.; Pérez, E. Manejo, integración y beneficios del biofertilizante micorrízico EcoMic® en la producción agrícola. R Rivera Ed Ediciones INCA, San José de las Lajas, Cuba. 2020.151p.
Buil, P.A.; Jansa, J.; Blažková, A.; Holubík, O.; Dufková, R.; Rozmoš, M.; Püschel, D.; Kotianová, M. & Janoušková, M. Infectivity and symbiotic efficiency of native arbuscular mycorrhizal fungi from high-input arable soils. Plant Soil. 2022.https://doi.org/10.1007/s11104-022-05715-8,
Liriano, R.; Núñez, D.B.; Barceló, R. Efecto de la aplicación de Rhizobium y micorriza en el crecimiento del frijol (Phaseolus vulgaris L) variedad CC-25-9 negro, Centro Agrícola, 39(4): 17-20. 2012.octubre-diciembre, ISSN papel: 0253-5785 ISSN on line: 2072-2001.
Martín, N. J.; Llerena, Á. B., Acosta, J.L. Influencia de los hongos micorrízicos arbusculares, abonos orgánicos y sacarosa en el cultivo del fríjol (Phaseolus vulgaris L.). Alternativas. 2018. ISSN: 13T90-1915, 19, (2), 89-96pp.
Bukovská, P., Rozmoš, M., Kotianová, M., Gancarcíková, K., Dudáš, M., Hršelová, H. & Jansa, J. Arbuscular Mycorrhiza Mediates Efficient Recycling From Soil to Plants of Nitrogen Bound in Chitin. Front. Microbiol. 2021.12:574060. doi:10.3389/fmicb.2021.574060,
Hidalgo, J. E.; Ramos C. C. Coinoculación de Rhizophagus irregularis y Rhizobium sp. en Phaseolus vulgaris L. var. canario (Fabaceae) “frijol canario”. Arnaldoa 26 (3): 991-1006. 2019. doi: http://doi.org/10.22497/ arnaldoa.263.26309..
Calero, A; Pérez, Y; Quintero, E; Olivera D. y Peña, K. Efecto de la aplicación asociada entre Rhizobium leguminosarum y microorganismos ef icientes sobre la producción del fríjol común. Cienc. Tecnol. Agropec. 2019. 20: 295-308. doi: https://doi.org/10.21930/rcta.vol20_num2_ art:1460.
Tamayo-Vélez, A.; Bernal Estrada, J. A. Common bean (Phaseolus vulgaris L.) yield response to chemical and biological fertilization in different localities of Colombia. Rev. Fac. Nac. Agron. Medellín 2018. 71: 8573-8579. doi: http:// dx.doi.org/10.15446/rfnam.v71n3.72392.
Colás-Sánchez, A.; Díaz-Pérez, B.; Rodríguez-Urrutia, A.; Gatorno-Muños, S. y Rodríguez-López, O. Efecto de la biofertilización en la morfo fisiología y rendimiento del frijol común (Phaseolus vulgaris L.). Revista Centro Agrícola.2018.45, (4), 34-42pp.