Spirulina extracts reverse the effects of simulated water deficit with PEG 6 000 in corn
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Abstract
The water deficit delays the germination and initial growth of maize seedlings and the application of biostimulants can reverse this effect. The objective of this research was to determine the effects of two ethanolic extracts of spirulina (Arthrospira platensis) on the germination and initial growth of maize seedlings subjected to simulated water deficit with PEG 6 000. Two experiments were performed using cultivar P-7928. At the first one, desinfected seeds were placed to germinate, during seven days, in water, PEG 6 000 15 % and PEG 6 000 15 % supplemented with different concentrations (1, 0.5 and 0.1 µL mL-1) of two ethanolic extracts of spirulina (SpE 1 y SpE 2). At the end of experiment, final percentage of germination and some indicators of seedling growth were evaluated. In the second one, the best performing spirulina extract was used with the same concentrations proceeded in a similar way to the first experiment. The germination dynamics was followed every 12 hours and final percentage of germination (% G), germination velocity (GV), mean germination time (MGT), germination rate (GR) were calculated. Results showed that the imposed osmotic stress delayed germination but did not affect the final percentage and decreased significantly the shoot length. The addition of an ethanolic extract of spirulina (SpE 2) at 1.0 µL mL-1 concentration completely reversed the delay in germination imposed by PEG 6000 and generally stimulated the seedling growth.
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