Phenotypical characterization of rhizobia isolates from forage legume Canavalia ensiformis
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Abstract
phenotypically rhizobia isolates from forage legume Canavalia
ensiformis. Twelve isolates (five possible members of the
Rhizobium genus, seven possible members of Bradyrhizobium
genus) and two commercial strains, BR 2001 and BR 2003a
were characterized. For the phenotypic characterization of the
bacterial isolates, their capacity to use six carbohydrates as
carbon source, their tolerance to acid condition and different
levels of NaCl, and the resistence to six antibiotic, were studied;
as well as their growing in three incubation temperatures. In
addition, the capacity of these isolates to produce
polyhidroxibutirates (PHB) was determinated. All rhizobia
isolates used lactose, glucose and mannitol as carbon source.
One rapid growing isolate was the most tolerant to pH 4.5. The
isolates possible members of the Rhizobium/Sinorhizobium
genus were more tolerant to NaCl than the isolates possible
members of Bradyrhizobium genus. Three of the rapid growing
isolates were resistent to six antibiotics. When the isolates
were incubated at 37
0
C, all of them except the CP3 isolate and
one commercial strain, BR 2001, grown similar to control. The
CP10 slow growing isolate had the capacity to produce the
biggest PHB concentration (0.023 g.L
-1
)
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