Indirect organogenesis in apical meristems from Cuban rice variety Reforma
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Abstract
to the more commonly used methods for in vitro cereal
regeneration, as they are less genotype-dependent, and more
efficiently established seedbeds can be used as donor mate-
rial. In the present work, de novo morphogenesis processes
were induced by indirect organogenesis in apical meristems
from in vitro rice plantlets of Reforma cultivar germinated on a
MS basal medium. Shoot meristems were obtained when cutting
three 2.0-cm segments from the bottom portion of seedling
stem. At callus phase, different concentrations of 2,4-D were
trialed in the culture medium, also testing the effect of stem
segment position on callus formation. In general, two types of
calli were observed: morphogenic and nonmorphogenic ones,
which differed in their structure and growth. The highest
proportion of morphogenic calli was obtained at the
bottommost stem segment, using 2.0 mg/L 2,4-D. These calluses
were subcultured for 10 days under light or dark conditions, to
study their effect on shoot regeneration. At the regeneration
media, macro and microelements from MS and N6 media were
evaluated separately. From subcultured calluses in dark
regenerated more shoots at the regeneration medium with MS
macro and microelements rather than with N6, whereas in
subcultured calluses in the light, shoot regeneration efficiency
diminished radically in both media tested.
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