In vitro propagation of macho plaintain (Musa sp. AAB) clone Sobrino by some Cuban bio-stimulants, BB-6 and Biostan, as growth regulator substitutes

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E. Héctor

Abstract

The possibility of using Cuban bio-stimulants,
as substitutes for conventional growth regulators in the full
propagation of bananas and plantains, would open a new
prospect for those biofactories at present devoted to Musa
genus multiplication in Cuba, and would also increase the exportable
potentials of Cuban biotechnological products. The
effects of Cuban bio-stimulants, BB-6 and Biostan, as potential
substitutes for growth regulators at the in vitro establishment,
multiplication and rooting stages, and their long-term
action on the acclimatization of macho plantain (Musa sp. AAB)
clone Sobrino were studied. At the establishment stage,
substituting indoleacetic acid for BB-6 or Biostan increased
explant survival, leaf number and explant length, without
affecting the number of days required to perform the first
subculture. At the multiplication stage, substitution did not
affect multiplication index, leaf number and explant length. The
addition of only bio-stimulants to the culture medium led to
lower values of the variables than the control in both phases.
At the rooting phase, substituting AIA for Biostan or BB-6
increased root number, average length, leaf number and
vitroplant length. The effect was kept for a long time in the
acclimatization phase, in which plants obtained with Biostan
or BB-6 showed higher values than those attained with AIA,
in all variables measured.

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How to Cite
Héctor, E. (2013). In vitro propagation of macho plaintain (Musa sp. AAB) clone Sobrino by some Cuban bio-stimulants, BB-6 and Biostan, as growth regulator substitutes. Cultivos Tropicales, 28(1), 13–18. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/332
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Original Article