Influence of high temperature on tomato (Lycopersicon esculentum Mill.) root tissues
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Abstract
of high temperature (38oC for 1.5 h) were studied in two tomato
(Lycopersicon esculentum Mill.) seedlings with different heat
tolerance degrees. Seven days after rice seeds started to
germinate, the heat treatment was applied to rootlets of each
variety, which were isolated in pots containing water and later
put on the stove for 1.5 h at 38oC, whereas the control treatment
consisted of leaving the other group of rootlets under
laboratory conditions while applying high temperature.
Afterwards, rootlet samples were taken and processed through
light microcopy, including epon resin and 2 ?m-wide sections
were cut and stained with toluidine blue, observed and
photographed under a microscope with a digital camera.
Longitudinal sections of rootlets showed that high temperature
increased vacuole size and expanded cell walls, among other
disorders; such changes became more evident at the heat stress
tolerant variety.
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