Review Optimization of culture media for microorganisms, a valuable strategy for agriculturally-interesting biopreparation production
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Abstract
microorganisms under laboratory conditions, trying to
simulate their natural habitat to satisfy their most vital needs
as living beings. The design of a culture medium responds to
demands of the microorganism in question and to the objective
pursued with its multiplication; therefore, the selection of
nutrients and their appropriate concentrations is a greatly
important factor. However, the employment of an appropriate
methodology makes possible the obtaining of an optimum
culture medium, either for growth and/or metabolite
production. Numerous methods and designs of optimization
exist with dissimilar characteristics, advantages and limitations
that determine their use in diverse branches of investigation.
The complete and fractional factorial designs, Plackett
Burman, Box-Behnken and the uniform designs were
approached in the work. The optimization of medium
components and environmental conditions guarantees
remarkable yield increments of fermentative processes in a
quick and economic way. Therefore, this review seeks to
approach the principles and methods of optimization, as well
as characterize and exemplify the application of most used
designs in culture media optimization, with the objective of
extending their use to agricultural biotechonology.
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