Short communication A methodology for spore germination of Glomus mosseae
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Abstract
processes during the life cycle of arbuscular mycorrhizal fungi
(AMF) and the successful symbiosis establishment depends
greatly on it. The objective of the present work was to evaluate
CO2 effect on the rate and homogeneity of Glomus mosseae
spore germination under in vitro conditions. An experiment
was established under a Randomized Complete Design with
bifactorial arrangement and three repetitions including the
study of different spore disinfection methods and germination
conditions, through the use of Cloramin T and antibiotics, and
a CO2 atmosphere (5 %) in the spore incubation chambers.
Results showed the positive effect of disinfection treatments
on spore germination and contamination percentages, the use
of a CO2 atmosphere during incubation being an important
requirement to get a full germination without detecting
contaminants in only three days of incubation. This study
permitted to reckon with a new methodology to guarantee a
quick and successful germination of G. mosseae spores in
absence of contamination, enabling the introduction of fungal
propagules as inoculum during plant micropropagation
process.
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