DNA polymorphisms in Cuban varieties of avocado (Persea americana Mill.) as detected by inverse sequence tagged repeat (ISTR) analysis
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Abstract
commercial avocado varieties was initiated using ISTR analysis.
Dice�??s dissimilarities between genotype pairs were calculated.
A cluster analysis was performed based on dissimilarity using
UPGMA as the clustering method. ISTR markers were efficient
in detecting polymorphisms among the genotypes. The
dissimilarity values ranged from 0.24 between var. �??Suardía�?�
and �??Hass�?� to 1.00 between �??Lula�?� and �??Los Moros�?� or �??CHI-
3�?� with an average dissimilarity of 0.78. The efficiency of
UPGMA in estimating genetic relationships between varieties
was corroborated by the cophenetic correlation coefficient,
which indicated that the distortion degree in the estimated
dissimilarities was minimal. Ecological groups were not
adequately represented in the dendrogram. Thus, West
Indians, Guatemalan and Mexican genotypes were positioned
across the dendrogram. The utility of ISTR for genotype
identification and assessment of genetic diversity in commercial
avocado varieties is discussed
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