In vitro mycorrhization of coffee (Coffea canephora var. Robusta) plantlets: Is it a fact?

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Kalyanne Fernández

Abstract

A survey of current literature reveals that
inoculation of arbuscular mycorrhizal fungi (AMF) in the roots
of micropropagated plantlets plays a beneficial role. The
objective of this investigation was to evaluate inoculation
effects of AMF Glomus clarum upon in vitro development of
coffee plantlets (Coffea canephora var. Robusta). Two types
of propagules (spores and external mycelium) and a
noninoculated control treatment were studied under a
Randomized Complete design, evaluating stalk height, leaf and
root fresh weight, leaf pair number, root number, microbial
contamination percentage, embryo counting and classification,
presence of fungal structures and enzymatic activities of
Peroxidase and Polyphenoloxidase. After 30 days of root
inoculation, plantlets showed higher altitude and more leaf
pair number and root number than noninoculated plants.
Besides, a new embryogenic callus production, was found in
these plants. Finally, significant differences were observed in
the enzymatic activities of inoculated plantlets, demonstrating
host presence inside roots.

Article Details

How to Cite
Fernández, K. (2013). In vitro mycorrhization of coffee (Coffea canephora var. Robusta) plantlets: Is it a fact?. Cultivos Tropicales, 23(3), 47–52. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/635
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Original Article