The effect of culture medium composition and genotype on somatic embryogenesis induction in Coffea canephora P. var. Robusta clones

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Published: May 31, 2013
Keywords:
Coffea canephora, robusta coffee, somatic embryo, callus, picloram

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María E. González
Instituto Nacional de Ciencias Agrícolas

Abstract

The investigation was developed in the Laboratory of Crop Genetics and Breeding from the National Institute of Agricultural Sciences (INCA) in the period between 1997 and 1998, with the objective of studying the influence of some factors during the process of somatic embryogenesis, among them the culture medium composition and genotype. Thus, vegetable material coming from the clones C-R, M-229, K-234 and M-28 of Robusta variety, Coffea canephora P., from the Central Research Station of Coffee and Cocoa was used. In order to study the effect of the culture medium, several hormonal combinations between Picloram 2,4-D and 6-BAP were tested. The variables evaluated were fresh weight (g), coloration, growth rate, consistency and total proteins in the calluses, as well as explants with high frequency embryogenic calluses. Significant differences for the studied treatments were obtained, the clone M-229 being the best answer and M-28 the most recalcitrant for the studied indicators. It was determined that Picloram at the concentrations of 0.5 and 1 mg.L-1 and combined with 2 mg.L-1 of 6 BAP enhances embryogenic callus formation in the materials evaluated.

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How to Cite
González, M. E. (2013). The effect of culture medium composition and genotype on somatic embryogenesis induction in Coffea canephora P. var. Robusta clones. Cultivos Tropicales, 22(1), 17–21. Retrieved from https://ediciones.inca.edu.cu/index.php/ediciones/article/view/709
Issue
Vol. 22 No. 1 (2001)
Section
Original Article

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